Diallyl sulfides—natural compounds abundant in garlic—extended lifespan and improved multiple aging markers in male mice through enhanced hydrogen sulfide production. The compounds increased protein persulfidation, a cellular signaling mechanism that modulated mTOR pathways and immune responses while reducing liver fat accumulation. Treated mice showed better glucose control, motor function, and cognitive performance across their lifespan. This represents a significant advance in aging research because it demonstrates that a specific gasotransmitter pathway can be pharmacologically targeted to improve healthspan. Previous longevity interventions like caloric restriction were known to boost hydrogen sulfide, but this is the first direct evidence that enhancing H₂S generation alone extends mammalian lifespan. The human validation data—showing that individuals with higher protein persulfidation had greater muscle strength and lower triglycerides—suggests this mechanism operates across species. However, the study was limited to male mice, and the optimal dosing and safety profile in humans remains unclear. The findings position hydrogen sulfide as a potentially druggable target for aging interventions, moving beyond correlational studies to demonstrate causation.