Researchers developed HELF (High Efficiency Labeling of Fibers), a protocol using the fluorescent aminosterol trodusquemine combined with glutaraldehyde fixation to visualize myelin sheaths in cleared nervous tissue. The approach overcomes a major technical barrier in neuroscience imaging, as traditional tissue clearing methods remove the lipids that compose myelin, making it nearly impossible to study nerve fiber structure in three dimensions. Trodusquemine's strong affinity for cholesterol-rich membranes allows it to bind specifically to myelin, producing bright fluorescent signals that remain stable during the clearing process. This breakthrough addresses a critical gap in multiple sclerosis research, where understanding myelin damage patterns throughout the spinal cord has been severely limited by imaging constraints. Previous immunolabeling attempts failed to provide adequate contrast or specificity for myelin visualization in cleared tissues. The HELF protocol successfully demonstrated whole spinal cord imaging in mice and showed promising results in human brain samples, potentially revolutionizing how researchers study demyelinating diseases. While the technique represents a significant technical advance, its clinical translation will require validation across larger sample sizes and different disease models to establish its reliability for therapeutic evaluation.